Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.185553 |
Chromosome: | chromosome 3 |
Location: | 6016858 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g190900 | (1 of 5) PTHR10351:SF42 - LD40663P | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAGCGCGTCTATAATCGCATGTATAGCA |
Internal bar code: | AGAACTACACCCATGCTTCTAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1355 |
LEAP-Seq percent confirming: | 99.5548 |
LEAP-Seq n confirming: | 5591 |
LEAP-Seq n nonconfirming: | 25 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGAGGAGACGGAGAAAATC |
Suggested primer 2: | CGAGGAACGCCTAGTGTCTC |