| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.185557 |
| Chromosome: | chromosome 6 |
| Location: | 305077 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g251100 | NMT1 | (1 of 1) 2.3.1.97 - Glycylpeptide N-tetradecanoyltransferase / Peptide N-tetradecanoyltransferase; N-myristoyl transferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGTGGGCGTGGGGGAGGTGGAGGCATT |
| Internal bar code: | TGTATCTCCTCGTTTACGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 860 |
| LEAP-Seq percent confirming: | 99.5893 |
| LEAP-Seq n confirming: | 970 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGCAGCTGGTGAATGAC |
| Suggested primer 2: | ACTTACTGGGTCTTGCCCCT |