Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.185568 |
Chromosome: | chromosome 1 |
Location: | 6936538 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g049950 | VLE1,SPO | Sporangin (Vegetative Lytic Enzyme 1); (1 of 14) 3.4.21.62 - Subtilisin | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTACCTATCGATCTGCAAGTGAAGTCTA |
Internal bar code: | GCGTGGGCAAGAGCACGGCTAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 827 |
LEAP-Seq percent confirming: | 22.7426 |
LEAP-Seq n confirming: | 539 |
LEAP-Seq n nonconfirming: | 1831 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGGATGAACGGCATGTTTT |
Suggested primer 2: | TACCCGACTGACTCGCTCTT |