| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.185568 |
| Chromosome: | chromosome_1 |
| Location: | 6936538 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g049950 | VLE1,SPO | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCTACCTATCGATCTGCAAGTGAAGTCTA |
| Internal bar code: | GCGTGGGCAAGAGCACGGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 827 |
| LEAP-Seq percent confirming: | 22.7426 |
| LEAP-Seq n confirming: | 539 |
| LEAP-Seq n nonconfirming: | 1831 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGATGAACGGCATGTTTT |
| Suggested primer 2: | TACCCGACTGACTCGCTCTT |