| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.185572 |
| Chromosome: | chromosome 12 |
| Location: | 2130676 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g508900 | MAPK6 | (1 of 3) K04371 - mitogen-activated protein kinase 1/3 (MAPK1_3); Mitogen-Activated Protein Kinase 6 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCAGTACTGGCAACAAACATGCCTCTAA |
| Internal bar code: | AATTACGCGCCGGAAGCCGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 305 |
| LEAP-Seq percent confirming: | 99.9263 |
| LEAP-Seq n confirming: | 1356 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCTCAAGCTCATCATCA |
| Suggested primer 2: | CGAGTCACAAGAACCTGCAA |