Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.185581 |
Chromosome: | chromosome_3 |
Location: | 5430852 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g185050 | FAP53 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGGAACACACAGTTTGTTAGAGCCGTGTAT |
Internal bar code: | GCGACGCGCAGTCTCGCGAGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 481 |
LEAP-Seq percent confirming: | 99.6694 |
LEAP-Seq n confirming: | 1206 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGTGTATGCGTTTGGAGGG |
Suggested primer 2: | TTCTCATCACGCAGAACGTC |