| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.185582 |
| Chromosome: | chromosome 4 |
| Location: | 678004 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217952 | (1 of 1) IPR013083//IPR026319 - Zinc finger, RING/FYVE/PHD-type // Zinc finger C2HC domain-containing protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGCCCGCGTAGCCGCCCGCCGCTGGGG |
| Internal bar code: | GCGGCTTTTTGGTGTACTTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 720 |
| LEAP-Seq percent confirming: | 99.5607 |
| LEAP-Seq n confirming: | 4079 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCTCGCTATCAACTTCC |
| Suggested primer 2: | GAGCTTGCTACTGGGTCCTG |