| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.185589 |
| Chromosome: | chromosome 7 |
| Location: | 1880224 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325739 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTCAGAACCGGGGCTCGTATACCTCGG |
| Internal bar code: | TAGGACTGCCGATGCGTCCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 930 |
| LEAP-Seq percent confirming: | 99.7673 |
| LEAP-Seq n confirming: | 3001 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGTGATGAGACCGTGTT |
| Suggested primer 2: | TGCCCCAAGACATAGTTTCC |