| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.185609 |
| Chromosome: | chromosome 1 |
| Location: | 4618606 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g031750 | (1 of 1) K11883 - RNA-binding protein NOB1 (NOB1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGATACAGCACTAGTTGGTTCTTCTACGG |
| Internal bar code: | GTGTAGGTGTTTACTATGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 440 |
| LEAP-Seq percent confirming: | 98.2315 |
| LEAP-Seq n confirming: | 611 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGAAACGGTCGCGTAGA |
| Suggested primer 2: | AGACCTGCAGGACATCATCC |