| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.185610 |
| Chromosome: | chromosome 2 |
| Location: | 2253016 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g090100 | (1 of 1) PTHR22883//PTHR22883:SF23 - ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN // PALMITOYLTRANSFERASE ZDHHC4-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCACCAATGCCCCCCATGCCCCCCTTC |
| Internal bar code: | AATTCGAGGGTTAACTTTCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 592 |
| LEAP-Seq percent confirming: | 98.5026 |
| LEAP-Seq n confirming: | 2105 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGGAGGAGTAGAGGACCC |
| Suggested primer 2: | AAATCAAACCAAACCAAGCG |