| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.185611 |
| Chromosome: | chromosome 12 |
| Location: | 4180098 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g518450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCGCACCCCAACATGTGTTGCGGGCGGT |
| Internal bar code: | ACTACTTTCGGGGTGTGGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 241 |
| LEAP-Seq percent confirming: | 98.4439 |
| LEAP-Seq n confirming: | 2657 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGGAGAAGATTGACCCAT |
| Suggested primer 2: | CAACTGTGTATCGCACACCC |