| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.185652 |
| Chromosome: | chromosome 6 |
| Location: | 8375740 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g306750 | FAP290 | Flagellar Associated Protein 290; (1 of 1) K13448 - calcium-binding protein CML (CML) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATTCTATCTATCTGAACTTAAGGCCAAG |
| Internal bar code: | AGACCACGCCGCCATCAGAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 600 |
| LEAP-Seq percent confirming: | 97.0 |
| LEAP-Seq n confirming: | 97 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTTTCCACGTAGGTAGGG |
| Suggested primer 2: | CGGGTACGGCCTTACACTTA |