| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.185653 |
| Chromosome: | chromosome 6 |
| Location: | 3357919 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277700 | (1 of 1) 2.7.1.108 - Dolichol kinase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCAGAAGAAGGAGTCCTTGATTGCAGC |
| Internal bar code: | AGCTTCGGACGCCTAGGCAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 879 |
| LEAP-Seq percent confirming: | 99.5741 |
| LEAP-Seq n confirming: | 1169 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGACAAAGGTGTGAATG |
| Suggested primer 2: | ACATCGTAAGGTGGTGGGAG |