| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.185667 |
| Chromosome: | chromosome 13 |
| Location: | 33819 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g562031 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATGAAATGTTGATGGACACTGCATGCAC |
| Internal bar code: | ACGGGCGTTCGCGTACGATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 477 |
| LEAP-Seq percent confirming: | 98.087 |
| LEAP-Seq n confirming: | 6153 |
| LEAP-Seq n nonconfirming: | 120 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCAATCTGAAAGCCATCC |
| Suggested primer 2: | TAGCGTGCTGACAACCTTTG |