| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.185673 |
| Chromosome: | chromosome 2 |
| Location: | 2080602 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g088900 | PRPL1 | Plastid ribosomal protein L1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGCGGCGGTGGCTTTGGGGTTCAGGTC |
| Internal bar code: | TTAACGCGTGTCGAGTGGTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1087 |
| LEAP-Seq percent confirming: | 99.7923 |
| LEAP-Seq n confirming: | 4324 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAACACTAAGGGCAAGGC |
| Suggested primer 2: | AATGCAGGGTGTTTCCAGAC |