Insertion junction: LMJ.RY0402.185703_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.185703
Chromosome:chromosome 9
Location:3233246
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g387689 FAP76,CCDC180 Flagellar Associated Protein 76; (1 of 1) PTHR21444//PTHR21444:SF14 - FAMILY NOT NAMED // COILED-COIL DOMAIN-CONTAINING PROTEIN 180 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTCTCGCACCCCTCGCGCGCTGTCCTGCT
Internal bar code:CCATATGGAAAATAAATGGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:363
LEAP-Seq percent confirming:99.376
LEAP-Seq n confirming:637
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GAATAAACTGCCCCACTCCA
Suggested primer 2:GTAACCTGCACACGTCCCTT