| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.185713 |
| Chromosome: | chromosome 1 |
| Location: | 3308877 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g020887 | (1 of 2) K06972 - uncharacterized protein (K06972) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAACCAAACGACCGACGACCCGGCCAGG |
| Internal bar code: | GGGCAGGCGATCGGTAAGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 376 |
| LEAP-Seq percent confirming: | 90.6257 |
| LEAP-Seq n confirming: | 4244 |
| LEAP-Seq n nonconfirming: | 439 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCACTGATTGCTTCTGCAC |
| Suggested primer 2: | AGCGAGAGCTAACAGGGGTT |