Insertion junction: LMJ.RY0402.185724_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.185724
Chromosome:chromosome 16
Location:1665095
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g654600 PHC56 (1 of 71) PF12499 - Pherophorin (DUF3707); Pherophorin-chlamydomonas homolog 56 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCCGGGCACCCGTCTAGGCGACTGCAGA
Internal bar code:GAACGCGTTGCAGTGTCGATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:421
LEAP-Seq percent confirming:56.8395
LEAP-Seq n confirming:1579
LEAP-Seq n nonconfirming:1199
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCCTACCTTACCACGCAC
Suggested primer 2:CGAGTACAGCAGCGTGGATA