| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.185725 |
| Chromosome: | chromosome 12 |
| Location: | 5385890 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g529850 | (1 of 6) PTHR10582 - TRANSIENT RECEPTOR POTENTIAL ION CHANNEL PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTCGAAGGCTGGGGCCCGACCCGCACTC |
| Internal bar code: | GCGGCGCCCCGACTCCAGCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1061 |
| LEAP-Seq percent confirming: | 99.823 |
| LEAP-Seq n confirming: | 3383 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACGGACAAGGTCCATACT |
| Suggested primer 2: | CGCAGAGAGCGGTTACTACC |