| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.185756 |
| Chromosome: | chromosome 10 |
| Location: | 2806998 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439250 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACCCTGGCTGATGGCGGGCACGCTCCTA |
| Internal bar code: | AACGAGGCTGTGACTTGGCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 859 |
| LEAP-Seq percent confirming: | 89.6578 |
| LEAP-Seq n confirming: | 3511 |
| LEAP-Seq n nonconfirming: | 405 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCTTCAAGATCTCTGGC |
| Suggested primer 2: | AGTTATTCTGCGTTGCGCTT |