| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.185758 |
| Chromosome: | chromosome_7 |
| Location: | 635931 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g316992 | sense | intron|outside_mRNA |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCTGCAGGAGAGCCTGGCGCCCCGCCCCGC |
| Internal bar code: | AAGGCGGATAACCCGGAGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1021 |
| LEAP-Seq percent confirming: | 96.6373 |
| LEAP-Seq n confirming: | 1207 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCGGTTTTCCTTCTCTTG |
| Suggested primer 2: | AAACAGATTGTTTGGGCTGG |