| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.185765 |
| Chromosome: | chromosome 6 |
| Location: | 2112459 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g265300 | CSS2 | Chlamydomonas specific family S protein; (1 of 7) IPR011048 - Cytochrome cd1-nitrite reductase-like, haem d1 domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCCCAGCAGGGCGCGGCCTCGGGCAGGG |
| Internal bar code: | GTTAGTGATCTGGCGCGGCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 858 |
| LEAP-Seq percent confirming: | 99.6985 |
| LEAP-Seq n confirming: | 992 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGGGGCCCATATTACTCT |
| Suggested primer 2: | ATAGCTGCATGCGTCTTCCT |