Insertion junction: LMJ.RY0402.185792_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.185792
Chromosome:chromosome 6
Location:1751403
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g262000 SNR5 SNARE-associated Golgi protein; (1 of 6) PF09335 - SNARE associated Golgi protein (SNARE_assoc) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTATGTCGACCGTCCGCGTCCGCTGCGC
Internal bar code:AAGTATAGGGAAGATTGAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:614
LEAP-Seq percent confirming:99.0057
LEAP-Seq n confirming:697
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGAGTAATGCCGAGTTGCTG
Suggested primer 2:ATACATGTAGCGAGCCGAGG