Insertion junction: LMJ.RY0402.185795_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.185795
Chromosome:chromosome 16
Location:3773649
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g689300 STPK24,PTK27,STK24 Serine/Threonine protein kinase; (1 of 10) PTHR23257//PTHR23257:SF520 - SERINE-THREONINE PROTEIN KINASE // IP11267P 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGATGATCGTGAATCCTCCCACACAGGAA
Internal bar code:TGTCCGCTCCAGGTAAACGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:937
LEAP-Seq percent confirming:99.2701
LEAP-Seq n confirming:1224
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TGCAATCTCGTGACTTCGAC
Suggested primer 2:TGTCCTTCACACTCCCACAG