| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.185804 |
| Chromosome: | chromosome 3 |
| Location: | 5835729 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g189200 | (1 of 1) PTHR13192//PTHR13192:SF3 - MY011 PROTEIN // METHYLMALONIC ACIDURIA AND HOMOCYSTINURIA TYPE D PROTEIN, MITOCHONDRIAL | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTGCGGGGCCCATTGCCCCGCGTGCCGA |
| Internal bar code: | CGTGTCGTTTTATCGTGGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1027 |
| LEAP-Seq percent confirming: | 99.5169 |
| LEAP-Seq n confirming: | 1030 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTGGTGTTGAGGAGGTG |
| Suggested primer 2: | ACTCCACGCCAATGGTAGAC |