| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.185820 |
| Chromosome: | chromosome 12 |
| Location: | 9255580 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAACAGGTGCAACACCCTAGCAAACTTG |
| Internal bar code: | ACTCCTTCTCCAAACTACGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 389 |
| LEAP-Seq percent confirming: | 98.8235 |
| LEAP-Seq n confirming: | 2688 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGATGCGTAACCAGGAGGT |
| Suggested primer 2: | GCAACAGTAGTGCAGCAAGC |