| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.185851 |
| Chromosome: | chromosome 17 |
| Location: | 1016251 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g703400 | (1 of 3) PTHR11207//PTHR11207:SF7 - RIBONUCLEASE III // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCACATGCTGGGCTGGTTCGCCGCCGC |
| Internal bar code: | TTTAGATCCGTCCCCGTGACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 565 |
| LEAP-Seq percent confirming: | 99.2901 |
| LEAP-Seq n confirming: | 1958 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGTCTTAGAGTGTGCGG |
| Suggested primer 2: | ATCGCTGTTGGCTTCGTAGT |