| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.185870 |
| Chromosome: | chromosome 2 |
| Location: | 3906704 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g096250 | FMO9 | Flavin-containing monooxygenase; (1 of 1) 1.14.13.182 - 2-heptyl-3-hydroxy-4(1H)-quinolone synthase / 2-heptyl-3,4-dihydroxyquinoline synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCATCCTCCACACGCTGCTGCTGCTCCC |
| Internal bar code: | TAGGGATCTTACAGGGTAGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 499 |
| LEAP-Seq percent confirming: | 99.5856 |
| LEAP-Seq n confirming: | 3845 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGTAGCAACTCGTGACAG |
| Suggested primer 2: | AAGCGGGGACTGTTAGGAAT |