| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.185880 |
| Chromosome: | chromosome 17 |
| Location: | 3184320 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g721950 | (1 of 1) PTHR11685:SF119 - E3 UBIQUITIN-PROTEIN LIGASE ARI2-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAGCAGTCCGACGATGCACCATACCTCT |
| Internal bar code: | ATGCGGTACCGCACCAAGCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 102 |
| LEAP-Seq percent confirming: | 1.88798 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 3118 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTAGAGGTGACGAAGTGC |
| Suggested primer 2: | GAGGTTCAGCAAGCACATGA |