| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.185903 |
| Chromosome: | chromosome 1 |
| Location: | 306684 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001750 | TIG1 | chloroplast trigger factor; (1 of 1) PTHR30560 - TRIGGER FACTOR CHAPERONE AND PEPTIDYL-PROLYL CIS/TRANS ISOMERASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGGTGCTTTGTCCACGAGTGACAATTGA |
| Internal bar code: | CGAGTGCGCCCGGGATATAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 333 |
| LEAP-Seq percent confirming: | 27.904 |
| LEAP-Seq n confirming: | 1756 |
| LEAP-Seq n nonconfirming: | 4537 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGTGCATGTCTGTGCT |
| Suggested primer 2: | AAACGGTCGTCATGTGTTGA |