| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.185904 |
| Chromosome: | chromosome 2 |
| Location: | 5219119 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105900 | (1 of 1) 3.1.1.7 - Acetylcholinesterase / True cholinesterase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACTAAAGCATCTCCGATACCGTGTATAG |
| Internal bar code: | ATACGCGTGTGCACTCAAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 786 |
| LEAP-Seq percent confirming: | 99.6157 |
| LEAP-Seq n confirming: | 2592 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTATCCCGACCGTAACGAG |
| Suggested primer 2: | GTACCGTAATGCAGGTGGCT |