Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.185907 |
Chromosome: | chromosome 12 |
Location: | 1935509 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g510650 | FBP2,FBP1 | Fructose-1%252C6-bisphosphatase, chloroplastic; (1 of 1) K03841 - fructose-1,6-bisphosphatase I (FBP, fbp) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATGGGGCACATGCAACCACCGCTCGACA |
Internal bar code: | TCGACTGACGGCAGCCCATCAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 998 |
LEAP-Seq percent confirming: | 99.6148 |
LEAP-Seq n confirming: | 2586 |
LEAP-Seq n nonconfirming: | 10 |
LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGCCAACGCGTTACATACC |
Suggested primer 2: | ACATGGACAGCCTGAAGGAC |