| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.185919 |
| Chromosome: | chromosome 11 |
| Location: | 2719181 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g477000 | SNRK2D | snRK1 family in Chlamydomonas, subgroup 2; (1 of 41) IPR000719//IPR011009 - Protein kinase domain // Protein kinase-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGTACGCGATGGGACTGAATTTCGCGCT |
| Internal bar code: | TGACGGCTGTGTCCTCATCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 991 |
| LEAP-Seq percent confirming: | 99.5452 |
| LEAP-Seq n confirming: | 26481 |
| LEAP-Seq n nonconfirming: | 121 |
| LEAP-Seq n unique pos: | 102 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTGTTCGTTGGTTGTGAT |
| Suggested primer 2: | CCTTGGACTGAAACGTGGTT |