| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.185967 |
| Chromosome: | chromosome 7 |
| Location: | 5799253 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g353230 | (1 of 8) PF03747 - ADP-ribosylglycohydrolase (ADP_ribosyl_GH) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTGGCTTGCACACTGTCGTAGGACGGG |
| Internal bar code: | AGCATACCGCCGCACTTTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 310 |
| LEAP-Seq percent confirming: | 99.6297 |
| LEAP-Seq n confirming: | 3767 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCACCTAGCCTCTGGCTGT |
| Suggested primer 2: | CACACCTTTTACCACACCCC |