| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.186016 |
| Chromosome: | chromosome 10 |
| Location: | 3154012 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441950 | LSM2 | U6 snRNA-associated Sm-like small nuclear riboprotein LSm2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAACGTTGGATGCCGCTAGGGTGGCCTCG |
| Internal bar code: | GCGTGAGAGGGGGCGCAGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 969 |
| LEAP-Seq percent confirming: | 99.4649 |
| LEAP-Seq n confirming: | 2788 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAGGTCTTTTATGGTGGC |
| Suggested primer 2: | TTCTGCTTTCATGTCGTTGC |