Insertion junction: LMJ.RY0402.186023_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.186023
Chromosome:chromosome 1
Location:3435016
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g021950 (1 of 1) PF08393//PF12777//PF12781 - Dynein heavy chain, N-terminal region 2 (DHC_N2) // Microtubule-binding stalk of dynein motor (MT) // ATP-binding dynein motor region D5 (AAA_9) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGCCTGCTCACGCCCCGCTACCCGCTAC
Internal bar code:GGAAGGATCTCGGGCCGACGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:140
LEAP-Seq percent confirming:99.4792
LEAP-Seq n confirming:191
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTGAGGAGGGAATACGTTGG
Suggested primer 2:GCACTCGGCTGTCTTTGAG