| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.186030 |
| Chromosome: | chromosome 6 |
| Location: | 1063481 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g256500 | (1 of 2) PTHR14187//PTHR14187:SF5 - ALPHA KINASE/ELONGATION FACTOR 2 KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACACCTGCACGCAGCGCAGACCAGCACG |
| Internal bar code: | CTGTCGGGAACCATCTCATCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 355 |
| LEAP-Seq percent confirming: | 89.7661 |
| LEAP-Seq n confirming: | 2149 |
| LEAP-Seq n nonconfirming: | 245 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCTCGTCGTATTCAGGAC |
| Suggested primer 2: | GTGACTCGCTCCAGTGCATA |