| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.186058 |
| Chromosome: | chromosome 7 |
| Location: | 6097692 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g355466 | AGE2 | DNA repair glycosylase; (1 of 1) PF15628 - RRM in Demeter (RRM_DME) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTACCGGGCCCTGTCCGTGTGCGTGCACC |
| Internal bar code: | GATAGCGGCTCGCAAAGAATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 304 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGGACCACTTTGCTGTCT |
| Suggested primer 2: | GGGCTACCGGATATGACCTT |