Insertion junction: LMJ.RY0402.186066_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.186066
Chromosome:chromosome 7
Location:4225119
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g342000 (1 of 1) PF00651//PF12847 - BTB/POZ domain (BTB) // Methyltransferase domain (Methyltransf_18) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCAATCACACAATCCTATCACATACACAC
Internal bar code:TGACCCACTATGGAGATTGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:265
LEAP-Seq percent confirming:98.9149
LEAP-Seq n confirming:1732
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GCCACACAACCAACTCACAC
Suggested primer 2:GCTCGGTTTAGCTTGGACTG