| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.186072 |
| Chromosome: | chromosome 9 |
| Location: | 3465035 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389430 | TSF3 | (1 of 1) PTHR11538:SF40 - PHENYLALANINE--TRNA LIGASE ALPHA SUBUNIT; Phenylalanyl-tRNA synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAGCCACAACGTGGCGACGTGCAGGACG |
| Internal bar code: | ATCGGCCCCCGGCCTAGGCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 317 |
| LEAP-Seq percent confirming: | 99.7809 |
| LEAP-Seq n confirming: | 2733 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGCTACTGCTGCTGCTC |
| Suggested primer 2: | CTGTTCTGCCCTGACCTCTC |