Insertion junction: LMJ.RY0402.186079_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.186079
Chromosome:chromosome 6
Location:1500753
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g259650 MPA7 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 1) PTHR10161 - TARTRATE-RESISTANT ACID PHOSPHATASE TYPE 5 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCGCCATGCACCCCCCGCTCACCAACCG
Internal bar code:TGGCGCCGATCATCCCCTAGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:578
LEAP-Seq percent confirming:99.8679
LEAP-Seq n confirming:1512
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTTGCAGAAGCAGTAGCG
Suggested primer 2:GCGAGTGCACTTCTACACCA