| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.186088 |
| Chromosome: | chromosome 2 |
| Location: | 1121850 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g081350 | (1 of 1) PTHR11080//PTHR11080:SF2 - PYRAZINAMIDASE/NICOTINAMIDASE // PROTEIN PNC-1, ISOFORM A; Nicotinamidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCGGCGGCTGCGGTGCTGTTGCTGCGA |
| Internal bar code: | TCGGTGAGGGGGGGCCAGAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1014 |
| LEAP-Seq percent confirming: | 92.4332 |
| LEAP-Seq n confirming: | 623 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCACCTTCCTCTTTCTGC |
| Suggested primer 2: | ACTTCCCATCCTGCAACAAC |