Insertion junction: LMJ.RY0402.186089_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.186089
Chromosome:chromosome 12
Location:4489974
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g521750 NUP133 Nucleoporin 133; (1 of 2) PF08801 - Nup133 N terminal like (Nucleoporin_N) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGGGCCCGTGCAAATGGCGTGTGCGAAG
Internal bar code:CCCACACATGCCGGCACCTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:1002
LEAP-Seq percent confirming:99.666
LEAP-Seq n confirming:2984
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCACACACACACACACAC
Suggested primer 2:GAACGACGAAGGGTTGGTTA