| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.186089 |
| Chromosome: | chromosome 12 |
| Location: | 4489974 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521750 | NUP133 | Nucleoporin 133; (1 of 2) PF08801 - Nup133 N terminal like (Nucleoporin_N) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCCTTGAACCCCCCACACAACAACCACG |
| Internal bar code: | ATGGGTTGTGTTTTTATACCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 107 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 117 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCACACACACACACACAC |
| Suggested primer 2: | GAACGACGAAGGGTTGGTTA |