| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.186090 |
| Chromosome: | chromosome 5 |
| Location: | 2314460 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234801 | (1 of 32) 3.1.1.3 - Triacylglycerol lipase / Triglyceride lipase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGCTCCTTCTGCCGGCAAGCCAAGTAAG |
| Internal bar code: | GCACACCAGCGTATCATTTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 608 |
| LEAP-Seq percent confirming: | 99.8459 |
| LEAP-Seq n confirming: | 648 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACCTCACTCCACTGCAA |
| Suggested primer 2: | TTCCTGTCTGTGCGTGTCTC |