| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.186097 |
| Chromosome: | chromosome 16 |
| Location: | 3549837 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679164 | (1 of 1) K15433 - RLL motif containing protein 1 (CGI99, CLE7, RLLM1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAACGCCAAGGCCACCCATGCTTTGAAG |
| Internal bar code: | GCGTAAAGATGGACGGCAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 610 |
| LEAP-Seq percent confirming: | 99.6758 |
| LEAP-Seq n confirming: | 1845 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCGAGCTAGGCAGAGAA |
| Suggested primer 2: | ACGTATGCACAACCGTTCAA |