| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.186099 |
| Chromosome: | chromosome 3 |
| Location: | 4962675 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g179961 | POLE1,POLE1-1 | (1 of 1) K02324 - DNA polymerase epsilon subunit 1 (POLE1); DNA polymerase epsilon subunit 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTTAAGGAAGTCGTAGACGGTAGTGGTC |
| Internal bar code: | CTTTCCGTTCATCAGGTACCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 262 |
| LEAP-Seq percent confirming: | 99.5411 |
| LEAP-Seq n confirming: | 2386 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGCGTGTAAGTGTGTGTA |
| Suggested primer 2: | GACAGCTTGAACTCCTTGCC |