| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.186119 |
| Chromosome: | chromosome 16 |
| Location: | 1579310 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g653750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGATGTGATTAGGAGCTGCCAGGCGTAGG |
| Internal bar code: | TACGGTGCAACTGCCACATATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 470 |
| LEAP-Seq percent confirming: | 96.8877 |
| LEAP-Seq n confirming: | 716 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTATCCCATCTTTTCCGT |
| Suggested primer 2: | GGTCAGGTAAACCCCATCCT |