| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.186120 |
| Chromosome: | chromosome_15 |
| Location: | 530197 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre15.g637050 | FAP274 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCTTACACATGGCGGTTTCGGTTTTTGCC |
| Internal bar code: | TACGCCCGCGCCGCGCGTCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 176 |
| LEAP-Seq percent confirming: | 99.2904 |
| LEAP-Seq n confirming: | 1679 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGAGTGCGTGTGCATGT |
| Suggested primer 2: | CCTCAGCTTACCTTCGTTCG |