| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.186120 |
| Chromosome: | chromosome 16 |
| Location: | 1370100 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651923 | CRI1,CRTISO1 | Carotenoid isomerase; (1 of 1) PTHR10668:SF5 - BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAAGAAAAAAGGCCCACGCGCAGTGAGG |
| Internal bar code: | CTGCTGTCCCGAGGGCGTCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 723 |
| LEAP-Seq percent confirming: | 99.3754 |
| LEAP-Seq n confirming: | 3182 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAACCCCTTCACTTGGTA |
| Suggested primer 2: | CTCAGATGTCTGACAGGGCA |