| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.186135 |
| Chromosome: | chromosome 16 |
| Location: | 5514882 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680600 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCCACCGTCTGTGACATGGAGTTGCGC |
| Internal bar code: | GGGGGCGGAATTCACGAAGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 366 |
| LEAP-Seq percent confirming: | 83.399 |
| LEAP-Seq n confirming: | 3175 |
| LEAP-Seq n nonconfirming: | 632 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTTTGGTGGGTGAGAGA |
| Suggested primer 2: | AATAGCGGCTCGTCAGCTC |